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CELL CULTURES

Academic year and teacher
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Versione italiana
Academic year
2022/2023
Teacher
GIANLUCA AGUIARI
Credits
2
Didactic period
Secondo Semestre
SSD
BIO/10

Training objectives

To learn cell culture techniques and their applications in dental research field.

Prerequisites

Notions of biochemistry, molecular biology and cell biology.

Course programme

Lessons:
Sterility conditions and culture media. Cell culture preparation. Cell proliferation and viability. Nucleic acid extraction. PCR . Real time RT-PCR. Western blotting and immunohistochemistry.
Practical lessons:
Cell culture preparation. PCR and protein electrophoresis.

Didactic methods

Lectures and practical training.

Learning assessment procedures

Oral exam divided in two parts
1) Discussion of a scientific report by using slides prepared by the student.
2) Two questions regarding course program.
Duration about 30 min.

Reference texts

Material provided by the teacher.
Terry A. Brown, Biotecnologie molecolari. Principi e tecniche, Zanichelli Ed.
Rob Reed David Holmes, Jonathan weyers, Allan Jones, Metodologie di base per le scienze biomolecolari, Zanichelli Ed.