OMICS AND MOLECULAR DIAGNOSTYC
Academic year and teacher
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- Versione italiana
- Academic year
- 2022/2023
- Teacher
- ILARIA LAMPRONTI
- Credits
- 6
- Curriculum
- Biotecnologie per la salute
- Didactic period
- Primo Semestre
- SSD
- BIO/10
Training objectives
- Omics are bio-molecular branches of life science, divided into several topics (genomics, transcriptomics, proteomics, metabolomics).
The principal objective of this course is make the students able to study the major techniques and instruments useful for the knowledge of novel omic analysis, essentially applied to a modern vision of early diagnosis. Students will be able to face the research of new diagnostic markers in clinical analysis. At the end of the lectures, in laboratory (12 hours), students may work individually on applicative and practical arguments. In this way students will be able to analyze the different protein expression starting from cultured cells and will have the possibility to apply modern analytical instruments. Prerequisites
- Extensive knowledge of biochemistry and molecular biology.
Course programme
- Introduction about the omic course and laboratory practice (1h)
PROTEOMICS and 2D GEL (4h)
-IEF technique
-SDS-PAGE technique
PEPTIDE MASS FINGERPRINTING (1h): techniques able to identify proteins using protein or genomic libraries.
CROMATHOGRAPHY, PROTEIN ARRAYS and HPLC Chip (2h): notes on modern techniques that support the classical proteomics
-HPLC: theory and diagnostic applications
-SELDI
-PROTEIN CHIP
LAB EMATHOLOGY (2h): common lab analysis and diagnosis of anemia (thalassemia, SCD: I, II and III level diagnosis)
MASS SPECTROMETRY (2h): techniques and applications in the proteome analysis: mass ionization methods (EI, CI, MALDI, ESI) and mass analyzers (quadrupole, ion trap, TOF).
IMMUNOLOGICAL ANALYSIS (3h): theory and diagnostic applications
-RIA, ELISA
-BIOPLEX-Luminex
BIOSENSORS in OMICS (2h): use of modern biosensors for clinical diagnosis
METABOLOMICS (2h): search of metabolites in biological fluids
-NMR technology
-GC/MS technology
EPIGENETICS/EPIGENOMICS (2h)
-DNA methylation and CpG islands
-Methylation Specific PCR (MSP)
CHROMATIN IMMUNOPRECIPITATION (2h)
-Immunopreciptation array
MICRORNAS (emerging role as biomarkers) (3h)
-Biogenesis of microRNAs
-Databases of microRNAs
-Therapeutical and diagnostic approach of microRNAs
TRANSCRIPTOMICS: Microarrays (2h)
INTERACTOME (2h)
GENOMIC ANALYSIS and FUNCTIONAL GENOMICS (2h): technologies and their applications, associated polimorfisms and pathologies, advanced genomic analysis, SNP’s arrays, linkage analysis and GWAS (genome-wide association study)
BIOBANK and DATABASES (2h)
DIAGNOSTIC BIOMARKERS (2h)
-Biomarkers in clinical practice (cancer markers, inflammation, etc.)
-Liquid biopsy biomarkers
-Prognostic and diagnostic relevance of circulating biomarkers
PRENATAL ANALYSIS (2h): non-invasive prenatal diagnosis (novel approaches and goals)
Laboratory practice will be based on:
1) Human immortalized cell line cultures.
2) Cell treatment with potential anti-inflammatory and anticancer agents.
3) Apoptosis analysis with AnnexinV assay (MUSE instrument) Didactic methods
- The course is organized in frontal lessons (40 hours) on the programme’s topics and in lab practice (12 hours). Weekly the lessons are presentations done with power point slides or real time search of web site database available online. During laboratory practice students will be divided in two groups and everyone will work individually.
Learning assessment procedures
- The exam is composed of a scientific relation of the laboratory experience and of an oral exam. The aim of the oral examination is to test the level of knowledge and deepening of the topics of the course program and the reasoning skills developed by the student on issues related to molecular diagnosis based on omic technologies. Generally, three arguments are asked, to which, in order to obtain the minimum grade, it is necessary to exhaustively respond. For each question 10/30 are attributed. The oral examination may finish also with one only question, if the commission verifies serious deficiencies of basic topics of the course.
Reference texts
- The power point slides of the lessons will be given to the student in PDF format in classroom.
The title of book chapters and articles from reviews or journals used as reference will be shown and cited during lessons.
