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BIOTECHNOLOGICAL DEFENSE AND AGRICULTURAL MICROBIOLOGY

Academic year and teacher
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Versione italiana
Academic year
2022/2023
Teacher
ELISABETTA CASELLI
Credits
12
Didactic period
Secondo Semestre

Training objectives

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Modulo: 57596 - DIFESA BIOTECNOLOGICA IN AGRICOLTURA
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BIOTECHNOLOGICAL DEFENSE
The aim of the course is to provide the current knowledge in the advances of plant protection in agriculture from pests. The course is divided into two parts. The first one is dedicated to the description of the different and putative target and barrier of insect pest. The second one is dedicated on the current techniques used for plant protection and the advanced methods with special attention to those with biotechnological background.

AGRICULTURAL MICROBIOLOGY
The course shall provide to the student knowledge of General Microbiology and Applied Microbiology in the principal aspects of the environmental Microbiology of interest in the agricultural field.
Knowledge and comprehension.
The student:
- shall know in detail the structure of the different microorganisms
- shall know in detail the microorganisms responsible for plant disease and the target of plant drugs or innovative methods
Capability of applying knowledge and comprehension.
The student:
- shall know how to use correctly the terminology of the applied microbiology
- shall be able to interpret the results of microbiological research

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Modulo: 72386 - MICROBIOLOGIA AGRARIA
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AGRICULTURAL MICROBIOLOGY
The course shall provide to the student knowledge of General Microbiology and Applied Microbiology in the principal aspects of the environmental Microbiology of interest in the agricultural field.
Knowledge and comprehension.
The student:
- shall know in detail the structure of the different microorganisms
- shall know in detail the microorganisms responsible for plant disease and the target of plant drugs or innovative methods
Capability of applying knowledge and comprehension.
The student:
- shall know how to use correctly the terminology of the applied microbiology
- shall be able to interpret the results of microbiological research

Prerequisites

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Modulo: 57596 - DIFESA BIOTECNOLOGICA IN AGRICOLTURA
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BIOTECHNOLOGICAL DEFENSE
The student must have basic knowledge of general entomology, and insect anatomy-physiology systems, molecular biology, genetics and ecology.

AGRICULTURAL MICROBIOLOGY
Knowledge of cell biology, biochemistry and molecular biology related to: structure and function of cell components, structure and synthesis of nucleic acids and proteins, differences between eukaryotic and prokaryotic cells, cell reproduction and metabolism.

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Modulo: 72386 - MICROBIOLOGIA AGRARIA
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AGRICULTURAL MICROBIOLOGY
Knowledge of cell biology, biochemistry and molecular biology related to: structure and function of cell components, structure and synthesis of nucleic acids and proteins, differences between eukaryotic and prokaryotic cells, cell reproduction and metabolism.

Course programme

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Modulo: 57596 - DIFESA BIOTECNOLOGICA IN AGRICOLTURA
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BIOTECHNOLOGICAL DEFENSE
The course consists of lectures, which cover the following topics:
Pest Management evolution, Conventional pest management, Integrated pest management IPM (pests monitoring, the economic thresholds in integrated pest management), Neurotoxic insecticides, Pheromone and mating disruption techniques, Midgut target-based insecticides, plants resistance to pests, breeding of plant resistance, transgenic plants for insect resistance (transgenic plants resistant to Bt toxin of Bacillus thuringiensis, transgenic plants expressing inhibitors of digestive enzymes of insects, transgenic plants expressing lectin), counter-resistance of pests to the transgenic plants, RNA interference, Growth and development target-based insecticides, Insect Immune system and Immune suppression toxins encoded by the parasitoid wasps. Sterile Insect Technique (SIT) and Incompatible Insect Technique (ITT) using Wolbachia. Mechanical and agronomic methods, the Natural control and the important role of biodiversity, Biological control (classical biological control, augmentative or propagative biological control), Microbiological pest control, (entomopathogenic viruses, fungi and bacteria). Integrated pest management of the most important pest present in Emilia-Romagna



AGRICULTURAL MICROBIOLOGY
The course consists of lectures, of about 2 hours each, covering the following topics:
Introduction to microbiology.
Morphology and cytology of microorganisms.
Nutrition and growth of microorganisms.
Microbial metabolism: catabolism and anabolism. Aerobic and anaerobic metabolism.
Metabolism regulation.
Microbial control.
Microbial taxonomy: bacteria, viruses and mycetes.
Prokaryotes (bacteria): Composition and structure of the bacterial cell. Bacterial metabolism, energy production and biosynthetic processes. Bacterial reproduction. Spore production. Bacterial culture. Genetic recombination processes. Special bacteriology. Chemotherapics and antibiotics. Drug resistance.
Virology: chemical composition, structure, and classification of viruses. Viral replication strategies. Virus colture and titration. Special virology.
Mycology: general features of mycetes and their classification. Special micology.
Applied microbiology: microbiology of food, industrial and environmental microbiology.
Alcoholic, lactic and acetic fermentation.
Relationship microorganisms-plants, microorganisms-soil, microorganisms-pollution.


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Modulo: 72386 - MICROBIOLOGIA AGRARIA
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AGRICULTURAL MICROBIOLOGY
The course consists of lectures, of about 2 hours each, covering the following topics:
Introduction to microbiology.
Morphology and cytology of microorganisms.
Nutrition and growth of microorganisms.
Microbial metabolism: catabolism and anabolism. Aerobic and anaerobic metabolism.
Metabolism regulation.
Microbial control.
Microbial taxonomy: bacteria, viruses and mycetes.
Prokaryotes (bacteria): Composition and structure of the bacterial cell. Bacterial metabolism, energy production and biosynthetic processes. Bacterial reproduction. Spore production. Bacterial culture. Genetic recombination processes. Special bacteriology. Chemotherapics and antibiotics. Drug resistance.
Virology: chemical composition, structure, and classification of viruses. Viral replication strategies. Virus colture and titration. Special virology.
Mycology: general features of mycetes and their classification. Special micology.
Applied microbiology: microbiology of food, industrial and environmental microbiology.
Alcoholic, lactic and acetic fermentation.
Relationship microorganisms-plants, microorganisms-soil, microorganisms-pollution.

Didactic methods

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Modulo: 57596 - DIFESA BIOTECNOLOGICA IN AGRICOLTURA
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BIOTECHNOLOGICAL DEFENSE
The course is structured in theoretical lectures for a total of 48 hours (6 CFU). The topics of the course will be described in depth in the classroom, moreover, references to scientific journals will also be presented. The topics covered will be explained through powerpoint presentations, which will be provided, in PDF format, to the students. Not all the topics of the course are covered by the recommended text books, they are therefore suggested for a better understanding of the basic concepts, it is therefore recommended to take part in the lessons


AGRICULTURAL MICROBIOLOGY
The course is structured in lectures for 48 total hours (6 CFU). All the topics included in the course will be described in depth during lectures, with the help of powerpoint presentations, which will be available to students. It is recommended to attend the lessons for effective comprehension of the microbiological phenomena.

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Modulo: 72386 - MICROBIOLOGIA AGRARIA
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AGRICULTURAL MICROBIOLOGY
The course is structured in lectures for 48 total hours (6 CFU). All the topics included in the course will be described in depth during lectures, with the help of powerpoint presentations, which will be available to students. It is recommended to attend the lessons for effective comprehension of the microbiological phenomena.

Learning assessment procedures

The final grade is a weighted average of the marks obtained in each of the two tests relative to the individual modules.

BIOTECHNOLOGICAL DEFENSE
The objective of the exam is to test the abilities of synthesis of the acquired knowledge and reasoning on the problems of the course.
Oral exam: the student will answer on average three-four general questions, followed by further questions that may range to other topics of the course. Above all, the exam will evaluate the ability of the student to correlate the different topics of the course and his/her knowledge.
The exams, whatever their form, might be also performed virtually, via PC, depending on the health emergency situation.

AGRICULTURAL MICROBIOLOGY
The objective of the exam is to test the acquired knowledge and the ability to reason on the studied topics.
Written test: the student shall answer to 31 multiple choice tests in 30 minutes of time.

Reference texts

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Modulo: 57596 - DIFESA BIOTECNOLOGICA IN AGRICOLTURA
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BIOTECHNOLOGICAL DEFENSE
Civolani S. Lotta ai fitofagi Un approccio farmacologico e biotecnologico, Prospettive e sfide, 2021, Tab edizioni
Notes and course material used during the classes.

AGRICULTURAL MICROBIOLOGY
Biavati, Sorlini: Microbiologia generale e agraria, Casa Editrice Ambrosiana.
Notes and course material used during the lectures.

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Modulo: 72386 - MICROBIOLOGIA AGRARIA
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AGRICULTURAL MICROBIOLOGY
Biavati, Sorlini: Microbiologia generale e agraria, Casa Editrice Ambrosiana.
Notes and course material used during the lectures.