BIOANALITYCAL CHEMISTRY
Academic year and teacher
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- Versione italiana
- Academic year
- 2022/2023
- Teacher
- MARIA CHIARA PIETROGRANDE
- Credits
- 6
- Didactic period
- Secondo Semestre
- SSD
- CHIM/01
Training objectives
- The main education aim is to provide to the students the basis knowledge of the main bioanalytical methods and their applications for qualitative and quantitative assays.
The main acquired knowledge will be:
- knowledge of the main bioanalytical methods involving biomolecules: immunochemical techniques, enzyme assay methods;
- knowledge of the basic properties of biosensors;
- knowledge of the most common applications of the main bioanalytical techniques for analysis of biological fluids and environmental samples.
The course will provide the proper abilities to the students in order to apply the obtained knowledge for solving a bioanalytical problem:
- ability in using immunochemical techniques and enzyme methods for identification and quantitative assays of analytes;
- to be able to choose a proper bioanalytical technique as the most useful for a specific analytical problem and to be able to motivate the choice. Prerequisites
- Basic knowledge of the instrumental methods for qualitative and quantitative analyses, contained in the Courses of Analytical Chemistry II and Laboratory of Analytical Chemistry II.
Course programme
- Chemical composition of body fluids (blood and urine), specimen collection and processing, sources of biological variation.
Spectrophotometric techniques: fluorimetry, nephelometry and turbidimetry: instrumentation and measurements.
Enzimatic methods for quantitation of enzyme activity and substrate concentration, immobilized enzymes.
Immunochemical techniques: antigen-antibody binding, qualitative and quantitative methods, competitive and non-competitive immunoassays, homogeneous and heterogeneous assays: EIA and ELISA assays. Enzyme assay methods: enzyme-substrate reactions.
Basic concepts and definitions of biosensors: biosensors based on enzymatic and immunochemical reactions using electrochemical and spectroscopic transducers.
Applications to environmental analysis: determinations of concentrations and toxicological effects of pollutants in environmental samples.
Applications to biochemical analysis: quali-quantitative determinations of proteins and enzymes in biological samples. Electrophoresis: basic principles of planar and capillary electrophoresis. Protein separation and analysis. Didactic methods
- Classroom lessons using PowerPoint slides.
Learning assessment procedures
- Oral examination with 3/4 questions on the characteristics of the described methods and on their applications to environmental and biological samples.
Reference texts
- The teacher will provide learning material: lecture notes used during the lessons and tutorial papers on the specific topics.
