CELLULAR BIOCHEMISTRY
Academic year and teacher
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- Versione italiana
- Academic year
- 2022/2023
- Teacher
- MARIA CRISTINA MANFRINATO
- Credits
- 6
- Didactic period
- Secondo Semestre
- SSD
- BIO/10
Training objectives
- The main goals of the course will be to provide students with knowledge on topics and issues related to the cellular biochemistry.
In particular, some aspects related to oxidative stress and enzymac processes in relation to neurodegenerative diseases and lysosomal storage disorders will be investigated.
At the end of the course the student must be able to perform calculations on the concentration of the solutions and to acquire specific skills on topics of the guided tutorials, including elaboration and analysis of results. Prerequisites
- Basic knowledge of chemistry and biochemistry
No specific propedeutical courses. Course programme
- The course forecasts 6 CFU of teaching divided in frontal lectures (4CFU) and guided tutorials in the lab (2CFU) with the following subjects:
Biochemistry of cell membranes; structure and function of lipids and membrane proteins. The erythrocyte membrane as a model of virus-cell interaction; the Sendai virus as an experimental model.
Lysosomal storage diseases and aspects of oxidative stress related to some diseases.
The cell: study of some aspects of oxidative stress and its modulators, in relation to various pathologies.
Neurodegenerative diseases such as Alzheimer's, TSE and prion-associated disease, particularly the prion protein’s misfolding
The Extracellular Matrix (ECM) with the involvement of Matrix Metalloproteases (MMPs) in Multiple Sclerosis (MS) pathogenesis.
The course aims, where it is possible, to highlight aspects sex/gender related.
Concentration of a solution, the ways to express the concentration and the methods of calculation.
Hemolysis and hemoagglutination assays.
Assay of Advanced Oxidation Protein Products (AOPP).
The assay of enzymes involved in lysosomal storage diseases in comparison to control enzymes.
Zymography of gelatinases, which are a class of MMPs involved in MS. Didactic methods
- The course is organized as follows:
Frontal lectures covering various topics in the program and guided tutorials in the lab where the students will do several experiments, ending with the elaboration and understanding of the results Learning assessment procedures
- The final exam consists of 20 multiple choice questions with three possible answers, which also includes the carrying out of calculations and the solution of a problem to test both the theoretical and practical skills acquired during the course. 11 questions are worth two points and 9 questions are worth one point; there are no penalties for incorrect answers and the proper resolution of the task allows to obtain the merit. The test will last 25 minutes.
Reference texts
- Teacher’s handouts on line in Google Classroom
