CELL CULTURES
Academic year and teacher
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- Versione italiana
- Academic year
- 2022/2023
- Teacher
- GIANLUCA AGUIARI
- Credits
- 2
- Didactic period
- Secondo Semestre
- SSD
- BIO/10
Training objectives
- To learn the basic cell culture techniques and their applications in dental research field.
Prerequisites
- Notions of Biochemistry, Molecular Biology and Cell Biology.
Course programme
- Theoretical lessons:
Sterility conditions and culture media. Cell culture preparation. Cell proliferation and viability. Nucleic acid extraction. PCR and Real time RT-PCR. Western blotting and immunohistochemistry.
Practical lessons:
Cell culture preparation. electrophoresis of proteins. PCR and electrophoresis. Didactic methods
- Lectures and practical training.
Learning assessment procedures
- Oral exam divided in two parts
1) Discussion of a scientific report by using slides prepared by the student.
2) Two questions regarding course program.
Duration about 30 min. Reference texts
- Material provided by the teacher.
Terry A. Brown, Biotecnologie molecolari. Principi e tecniche, Zanichelli Ed.
Rob Reed David Holmes, Jonathan weyers, Allan Jones, Metodologie di base per le scienze biomolecolari, Zanichelli Ed.
