MICROBIOLOGY
Academic year and teacher
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- Versione italiana
- Academic year
- 2022/2023
- Teacher
- DARIO DI LUCA
- Credits
- 12
- Didactic period
- Primo Semestre
- SSD
- MED/07
Training objectives
- The Microbiology course aims to provide the theoretical and practical knowledge and useful tools to prepare the student to recognize the composition, structure and biology of viruses, bacteria, fungi and protozoa. It aims to make the student acquire the knowledge of pathogenic microorganisms and the diseases they support. Furthermore, it aims to provide the student with the knowledge bases and the fundamental concepts to understand the mechanisms by which these organisms can cause diseases and the interactions between microorganisms and the infected host. Its objective is to make known and use the means available for the laboratory diagnosis of diseases supported by microorganisms.
In the discussion of some topics of the program, the principles of translational research will be presented.
As for the parameters of:
Know:
At the end of the course the student will know:
- the main microbiological infectious agents responsible for human pathology
- the basics of microbial, fungal and viral pathogenesis
- the interactions between microorganisms and host and the host response to infections
- the basics of antibacterial, antifungal and antiviral therapy
- the main laboratory procedures for the study and correct identification of microorganisms and viruses and their in vitro sensitivity to antimicrobial and antiviral drugs.
Know-how:
At the end of the course the student:
- Will be able to orientate in the field of bacterial, mycotic and viral pathology for systems and organs
- Will be able to indicate which laboratory analyzes are most appropriate for identifying pathogenic microorganisms
- Will be able to implement a rational approach aimed at the diagnosis and treatment of infections. Prerequisites
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Modulo: 007234 - MICROBIOLOGIA CLINICA
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Basic knowledge of biology, biochemistry and molecular biology Course programme
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Modulo: 007234 - MICROBIOLOGIA CLINICA
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BACTERIOLOGY: Composition and structure of bacteria. Metabolism. Spore. Cultivation. Genetic recombination. Bacterial pathogenesis. Toxins. Defenses of the organism. Serological reactions. Vaccines and serums. Chemotherapy and antibiotics. Bacteriological laboratory diagnostics. Staphylococci. Streptococci. Pneumococci. Neisserie. Corynebacterium. Enterobacteriaceae. Campylobacter and Helicobacter. Pseudomonas. Vibrio. Pasteurelle. Brucella. Emofili. Bordetella. Legionella. Bacilli. Anthrax. Clostridia. Mycobacteria. Borrelia. Leptospira. Treponemes. Mycoplasma. Rickettsia. Chlamydiae. Actinomycetes. Anaerobic bacteria.
VIROLOGY: Composition and structure of viruses. Classification. Viral replication. Cultivation and titration of viruses. Virus genetics. Viral pathogenesis. Viral oncogenesis. Antiviral defenses of the organism. Antiviral and interferon drugs. Virological laboratory diagnostics. Poxvirus. Herpesvirus. Adenovirus. Parvovirus. Polyomavirus. Papilloma virus. Reovirus. Paramyxovirus. Orthomyxovirus. Rhabdovirus. Togavirus. Picornavirus. Arbovirus. Coronavirus. Virus etiological agents of hepatitis. Retrovirus. AIDS. Emerging viral infections. Prions.
MYCOLOGY: Cytology and morphology, metabolism, sexual and asexual reproduction. Pathogenic action. Mycotoxins. Host immune response. Antifungal drugs. Cultivation of fungi. Principles of mycological diagnostics. Superficial mycoses: pityriasis. Skin mycoses: dermatophytes. Subcutaneous mycoses: sporotrichosis, chromoblastomycosis, mycetomas. Systemic mycoses: histoplasmosis, blastomycosis, coccidioidomycosis, paracoccidioidomycosis, cryptococcosis. Opportunistic mycoses: candidiasis, aspergillosis, zygomycosis, pneumocystis carinii.
PARASITOLOGY: General characters of protozoa. Amoebas. Hemoflagellates: leishmanias and trypanosomes. Intestinal and genitourinary flagellates: giardia, trichomonas. Plasmodium. Toxoplasma.
CLINICAL MICROBIOLOGY: General information on the main morbid pictures, and on the diagnosis of diseases of microbial origin of the various systems.
Isolation procedures and methods and identification of the various pathogenic microbial agents associated with infections of the following districts: cutaneous, respiratory, urogenital, gastrointestinal, nervous and circulatory. Sepsis.
LABORATORY TECHNIQUES: Microscopy, immunoenzymatic techniques. Critical considerations on the interpretation and meaning of laboratory tests. Didactic methods
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Modulo: 007234 - MICROBIOLOGIA CLINICA
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The course provides frontal lectures and online assisted teaching. The on-line teaching hours of moodle platform certification service consist in the deepening of some topics presented during the frontal lessons. Learning assessment procedures
- The examination will be carried out through a written exam lasting 20 minutes without using notes or books or multimedia supports, in the presence of teachers. 32 multiple-choice questions will be formulated (4 options for each question) and related to all training objectives. Each correct answer will be evaluated 1.0 point and there will be no penalty for incorrect or non-given answers. The final score is given out of thirty. The minimum mark for passing the exam, equal to 18/30, is obtained by those who answer exactly 18 questions out of 32 and the maximum mark of 30 cum laude will be attributed to those who pass the score of 30.
Reference texts
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Modulo: 007234 - MICROBIOLOGIA CLINICA
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- Murray et al., Microbiologia Medica, VIII edizione, EDRA
- Sherris, Microbiologia Medica, VI edizione, EMSI
- Antonelli et al., Principi di Microbiologia Medica, III ed, Casa Editrice Ambrosiana
