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TECHNIQUES OF MICROBIOLOGY

Academic year and teacher
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Versione italiana
Academic year
2022/2023
Teacher
VALENTINA GENTILI
Credits
6
Didactic period
Secondo Semestre

Training objectives

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Modulo: 007234 - MICROBIOLOGIA CLINICA
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Being able to successfully perform the most common techniques of microbiological laboratory.

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Modulo: 53792 - TECNICHE DI MICROBIOLOGIA CLINICA
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Being able to successfully perform the most common techniques of microbiological laboratory.

Prerequisites

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Modulo: 007234 - MICROBIOLOGIA CLINICA
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Principles of basic techniques utilized to study microbes and serological techniques.

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Modulo: 53792 - TECNICHE DI MICROBIOLOGIA CLINICA
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Principles of basic techniques utilized to study microbes and serological techniques.

Course programme

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Modulo: 007234 - MICROBIOLOGIA CLINICA
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Cell cultures laboratory: working in laminar flow hood, trypsinization, cell counting, monolayer cell cultures. Viral infection. Observation of the virus-induced CPE. Determination of titer of the virus by a count of the plaque produced or by hemagglutination. Gene Transfection. Use of fluorescence microscopy. Virological diagnosis with PCR and rt-PCR. Methods in Diagnostic Microbiological Laboratory : bacterial and viral gastroentheritis, Helicobacter pylori infections, diseases of the upper and lower respiratory system, microbial diseases of the urinary and reproductive systems.
Test for microbial susceptibility to antimicrobial drugs: Bauer and Kirby's test, MIC, E-test. Mycobacteria: microscopy, isolation and identification. Fungi: microscopy, isolation and identification. Parasitology: collection, transport systems and handling of samples. Serological diagnosis: TORCH, HIV, Syphilis.

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Modulo: 53792 - TECNICHE DI MICROBIOLOGIA CLINICA
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How to work in Cell culture laboratory: working in laminar flow hood, trypsinization, cell counting, monolayer cell cultures. Viral infection. Observation of the virus-induced CPE. Determination of titer of the virus by a count of the plaque produced or by hemagglutination. Gene Transfection. Use of fluorescence microscopy. Virological diagnosis with PCR and rt-PCR. Methods in Diagnostic Microbiological Laboratory : bacterial and viral gastroentheritis, Helicobacter pylori infections, diseases of the upper and lower respiratory system, microbial diseases of the urinary and reproductive systems.
Test for microbial susceptibility to antimicrobial drugs: Bauer and Kirby's test, MIC, E-test. Mycobacteria: microscopy, isolation and identification. Fungi: microscopy, isolation and identification. Parasitology: collection, transport systems and handling of samples. Serological diagnosis: TORCH, HIV, Syphilis.

Didactic methods

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Modulo: 007234 - MICROBIOLOGIA CLINICA
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Lectures and practical demonstrations

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Modulo: 53792 - TECNICHE DI MICROBIOLOGIA CLINICA
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Lectures and laboratory demontrations

Learning assessment procedures

Written Exam: multiple choice test

Reference texts

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Modulo: 007234 - MICROBIOLOGIA CLINICA
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Handout provided by the teacher.
G. Federici, S. Bernardini, A. Bertoli, P. Cipriani, C. Cortese, A. Fusco, P. Ialongo, C. Milani, Medicina di Laboratorio, McGraw-Hill Companies, Srl Publishing Group Italia
Saverio Simeone, Laboratorio di Microbiologia: corso pratico di tecnica ed analisi microbiologica, Editrice San Marco

------------------------------------------------------------
Modulo: 53792 - TECNICHE DI MICROBIOLOGIA CLINICA
------------------------------------------------------------
Handout provided by the teacher.
G. Federici, S. Bernardini, A. Bertoli, P. Cipriani, C. Cortese, A. Fusco, P. Ialongo, C. Milani, Medicina di Laboratorio, McGraw-Hill Companies, Srl Publishing Group Italia
Saverio Simeone, Laboratorio di Microbiologia: corso pratico di tecnica ed analisi microbiologica, Editrice San Marco