Salta ai contenuti. | Salta alla navigazione

Strumenti personali

ANATOMIC PATHOLOGY AND PATHOLOGICAL HISTOLOGY

Academic year and teacher
If you can't find the course description that you're looking for in the above list, please see the following instructions >>
Versione italiana
Academic year
2022/2023
Teacher
GIOVANNI LANZA
Credits
7
Didactic period
Secondo Semestre

Training objectives

------------------------------------------------------------
Modulo: 53785 - TECNICHE DI ANATOMIA PATOLOGICA
------------------------------------------------------------
The integrated course of Anatomic Pathology includes the modules of "Anatomic Pathology", " Anatomic Pathology Techniques" and " Cytopathology and Molecular Pathology" .
The Anatomic Pathology module aims to illustrate principles, aims and methods of modern anatomic pathology and aims to provide knowledge on main neoplasms' classification, diagnosis and staging and on prognostic and predictive pathologic characterization. The Anatomic Pathology Techniques module aims to provide knowledge on histological, histochemical, immunohistochemical and molecular morphology techniques employed in diagnostic surgical pathology and to provide basic knowledge on electron microscopy. The Cytopathology and Molecular Pathology module aims to provide knowledge on techniques employed in diagnostic cytopathology and molecular pathology.

Knowledge and understanding:
At the end of the course the student will:

-know classification and diagnostics of the main neoplasms of various organs and systems
-know the principles and purposes of the techniques used for processing histological and cytological samples in the pathology lab
- understand the rationale behind the use of immunohistochemistry and molecular biology in pathology
-know the principles and purposes of the immunohistochemical and molecular biology techniques used in the pathology lab

Applying knowledge and understanding:
At the end of the course the student will have developed the ability to:

- perform preparation of histological and cytological specimens
- to carry out the pre-analytical preparation of samples for molecular analysis;

------------------------------------------------------------
Modulo: 53786 - CITOPATOLOGIA E PATOLOGIA MOLECOLARE
------------------------------------------------------------
The integrated course of Anatomic Pathology includes the modules of "Anatomic Pathology", " Anatomic Pathology Techniques" and " Cytopathology and Molecular Pathology" .
The Anatomic Pathology module aims to illustrate principles, aims and methods of modern anatomic pathology and aims to provide knowledge on main neoplasms' classification, diagnosis and staging and on prognostic and predictive pathologic characterization. The Anatomic Pathology Techniques module aims to provide knowledge on histological, histochemical, immunohistochemical and molecular morphology techniques employed in diagnostic surgical pathology and to provide basic knowledge on electron microscopy. The Cytopathology and Molecular Pathology module aims to provide knowledge on techniques employed in diagnostic cytopathology and molecular pathology.

Knowledge and understanding:
At the end of the course the student will:

-know classification and diagnostics of the main neoplasms of various organs and systems
-know the principles and purposes of the techniques used for processing histological and cytological samples in the pathology lab
- understand the rationale behind the use of immunohistochemistry and molecular biology in pathology
-know the principles and purposes of the immunohistochemical and molecular biology techniques used in the pathology lab

Applying knowledge and understanding:
At the end of the course the student will have developed the ability to:

- perform preparation of histological and cytological specimens
- to carry out the pre-analytical preparation of samples for molecular analysis;

------------------------------------------------------------
Modulo: 56915 - ANATOMIA E ISTOLOGIA PATOLOGICA
------------------------------------------------------------
The integrated course of Anatomic Pathology includes the modules of "Anatomic Pathology", " Anatomic Pathology Techniques" and " Cytopathology and Molecular Pathology" .
The Anatomic Pathology module aims to illustrate principles, aims and methods of modern anatomic pathology and aims to provide knowledge on main neoplasms' classification, diagnosis and staging and on prognostic and predictive pathologic characterization. The Anatomic Pathology Techniques module aims to provide knowledge on histological, histochemical, immunohistochemical and molecular morphology techniques employed in diagnostic surgical pathology and to provide basic knowledge on electron microscopy. The Cytopathology and Molecular Pathology module aims to provide knowledge on techniques employed in diagnostic cytopathology and molecular pathology.

Knowledge and understanding:
At the end of the course the student will:

-know classification and diagnostics of the main neoplasms of various organs and systems
-know the principles and purposes of the techniques used for processing histological and cytological samples in the pathology lab
- understand the rationale behind the use of immunohistochemistry and molecular biology in pathology
-know the principles and purposes of the immunohistochemical and molecular biology techniques used in the pathology lab

Applying knowledge and understanding:
At the end of the course the student will have developed the ability to:

- perform preparation of histological and cytological specimens
- to carry out the pre-analytical preparation of samples for molecular analysis;

Prerequisites

------------------------------------------------------------
Modulo: 53785 - TECNICHE DI ANATOMIA PATOLOGICA
------------------------------------------------------------
Adequate knowledge of anatomy, biochemistry, histology and general pathology.

------------------------------------------------------------
Modulo: 53786 - CITOPATOLOGIA E PATOLOGIA MOLECOLARE
------------------------------------------------------------
Adequate knowledge of anatomy, biochemistry, histology and general pathology.

------------------------------------------------------------
Modulo: 56915 - ANATOMIA E ISTOLOGIA PATOLOGICA
------------------------------------------------------------
Adequate knowledge of anatomy, biochemistry, histology and general pathology.

Course programme

------------------------------------------------------------
Modulo: 53785 - TECNICHE DI ANATOMIA PATOLOGICA
------------------------------------------------------------
TECHNIQUES OF ANATOMIC PATHOLOGY

PART 1
Tissue preparation: Fixation, processing and inclusion, sectioning and preparation of sections on the slide. Histo-morphological and histochemical stains. Principles and methodologies of immunohistochemical techniques. Principles of transmission electron microscopy (TEM) and scanning electron microscopy (SEM), observation parameters for sample analysis and diagnostic applications.

PART 2
Organizational aspects of a Pathological Anatomy laboratory. Pre-analytical, analytical and post-analytical phase. Accreditation protocols and clinical risk management. Principles and methodologies of in situ hybridization techniques (FISH, CISH, SISH). Intraoperative histological examination. Automatic immunostainer: characteristics and operation. Role of the TSLB in the dissecting room.

------------------------------------------------------------
Modulo: 53786 - CITOPATOLOGIA E PATOLOGIA MOLECOLARE
------------------------------------------------------------
--

------------------------------------------------------------
Modulo: 56915 - ANATOMIA E ISTOLOGIA PATOLOGICA
------------------------------------------------------------
ANATOMIC PATHOLOGY
Definition, purpose and role of Anatomic Pathologicy. Procedures employed in pathology. Types of samples for histological and cytological examinations. Purpose and methods of intraoperative examination. Preparation and storage of samples for histological examination. Macroscopic examination and sampling of excisional biopsies and surgical specimen. TNM staging. Sentinel lymph node. OSNA. Principles and diagnostic applications of immunohistochemistry. Prognostic and predictive immunohistochemistry. Classification and diagnostics of the main neoplasms of various organs and systems.
TECHNIQUES OF ANATOMIC PATHOLOGY
PART 1
Tissue preparation: fixation, samples processing and inclusion, sectioning and preparation of slide sections. Histomorphological and histochemical stains. Principles and methodologies of immunohistochemical techniques. Principles of transmission electron microscopy (TEM) and scanning electron microscopy (SEM); observation parameters for sample analysis and diagnostic applications.
PART 2
Organizational aspects of a Pathological Anatomy lab. Preanalytical, analytical and post-analytical phase. Accreditation protocols and clinical risk management. Principles and methodologies of in situ hybridization techniques (FISH, CISH, SISH). Automatic immunostainer: characteristics and functioning. Role of the TSLB in dissecting room.
CYTOPATHOLOGY AND MOLECULAR PATHOLOGY
SECTION 1
Organization of the Molecular Pathology lab. Pre-analytical phase and sample enrichment techniques. Nucleic acids extraction. Techniques of
nucleic acids investigation. Main molecular tests in Anatomic pathology diagnostics Molecular diagnostics in lung cancer, large bowel carcinoma, breast
carcinoma, malignant melanoma and gastrointestinal stromal tumors (GIST). Quality assurance in molecular diagnostics.
SECTION 2
Sampling, enrichment, preparation and staining techniques in diagnostic cytopathology. Liquid-based cytology techniques. Immunocytochemical and molecular assays in diagnostic cytopathology. Cytologic features of malignant neoplasm. Reactive cellular changes. Computer assisted cytology. Diagnostic cytopathology: cervico-vaginal cytology, urinary cytology, bronchopulmonary cytology. Strategies and purposes of cancer screening programmes with particular reference to cervical cancer screening programme based on pap test and HPV test.

Didactic methods

------------------------------------------------------------
Modulo: 53785 - TECNICHE DI ANATOMIA PATOLOGICA
------------------------------------------------------------
The Anatomic Pathology Techniques module provides frontal lectures and practical activity in the Pathology lab.

------------------------------------------------------------
Modulo: 53786 - CITOPATOLOGIA E PATOLOGIA MOLECOLARE
------------------------------------------------------------
--

------------------------------------------------------------
Modulo: 56915 - ANATOMIA E ISTOLOGIA PATOLOGICA
------------------------------------------------------------
The Anatomic Pathology module provides frontal lectures.
The Anatomic Pathology Techniques module provides frontal lectures and practical activity in the Pathology lab.
The Cytopathology and Molecular Pathology module provides frontal lectures and practical activity in the Molecular pathology lab.

Learning assessment procedures

ANATOMIC PATHOLOGY
Oral exam: it consists, on average, of two or three questions on various topics and lasts 15-20 minutes.
TECHNIQUES OF ANATOMIC PATHOLOGY
1) Test in progress for fixation, histomorphological and histochemical methods, through preparation of a power-point presentation.
2) Written exam: six open questions
3) Oral exam: it consists, on average, of two or three questions on various topics and lasts 15-20 minutes
CYTOPATHOLOGY AND MOLECULAR PATHOLOGY
Oral exam: it consists, on average, of two or three questions on various topics and lasts 15-20 minutes.

In all the exams control of the topic, completeness of the information and the exhibition clarity will be evaluated.
The final vote is a weighted average of the marks obtained in each exam; the final outcome will depend also on the practice tests.

Reference texts

------------------------------------------------------------
Modulo: 53785 - TECNICHE DI ANATOMIA PATOLOGICA
------------------------------------------------------------
Didactic material of the course
Maria Rosaria Cardillo, Nozioni di tecniche diagnostiche di Anatomia Patologica, Antonio Delfino Editore
L.Ruco A.Scarpa, Anatomia Patologica. Le basi. UTET. Scienze mediche, 2007.

------------------------------------------------------------
Modulo: 53786 - CITOPATOLOGIA E PATOLOGIA MOLECOLARE
------------------------------------------------------------
--

------------------------------------------------------------
Modulo: 56915 - ANATOMIA E ISTOLOGIA PATOLOGICA
------------------------------------------------------------
Teaching material presented during frontal lessons will be provided on Classroom.
Maria Rosaria Cardillo, Nozioni di tecniche diagnostiche di Anatomia Patologica, Antonio Delfino Editore
L.Ruco A.Scarpa, Anatomia Patologica. Le basi. UTET. Scienze mediche, 2007.