SEPARATIVE METHODS
Academic year and teacher
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- Versione italiana
- Academic year
- 2022/2023
- Teacher
- MARTINA CATANI
- Credits
- 6
- Didactic period
- Secondo Semestre
- SSD
- CHIM/01
Training objectives
- The aim of the course is to understand the use of the main separation techniques used in the chemical and clinical-biological fields. The student will learn the theoretical foundations, the operating principles, the instrumentation and the most common applications of the following analytical methods: chromatography, capillary electrophoresis and mass spectrometry.
Prerequisites
- Basic knowledge of analytical chemistry, solution equilibria, spectrophotometric analysis and chromatography. Although no formal propedeuticity is required, students must have acquired and assimilated the content of the "Analytical Chemistry" course.
Course programme
- - Introduction to separative methods.
- Chemical separations based on extractions and crystallization.
- Chemical separations based on chromatography. Types of chromatography. Theory of chromatographic processes. Kinetic (efficiency) and thermodynamic (adsorption isotherms) aspects.
- Gas chromatography (GC). Instrumentation. Factors affecting GC separation. Mobile phases and stationary phases. Detectors. Applications.
- High performance liquid chromatography (HPLC). Instrumentation. Factors affecting a separation in HPLC. Mobile phases and stationary phases. Partition and adsorption chromatography. Ion exchange chromatography. Size exclusion chromatography. Affinity chromatography. Detectors. Applications.
- Supercritical Fluid Chromatography (SFC). Principles and instrumentation. Applications.
- Chiral chromatography. Main chiral stationary phases. Applications.
- Preparative chromatography. Principles and instrumentation. Applications.
- Mass spectrometry. Principles and instrumentation. Applications.
- Electrophoresis. Principles and instrumentation. Applications. Didactic methods
- The course is organized in 36 hours (6 CFU) of videorecorded lectures and focus group meetings. The course also includes exercises in order to promote learning.
Learning assessment procedures
- The exam is a written test with a multiple-choice questionnaire lasting 30 minutes. The test will contain 16 questions about all the topics discussed during the course; 2 points will be given for each right answer. The final evaluation is the totality of the correct answers indicated and a minimum score of 18/32 is required to pass the exam. The result of 32 will be assessed as 30 cum laude.
Reference texts
- Slides and material provided by the teachers.
The following textbooks are also recommended for consultation:
- D.C. Harris, "Chimica analitica quantitativa", Zanichelli, 3° edition, 2017.
- D.A. Skoog, D.M. West, F.J. Holler, S.R. Crouch "Fondamenti di Chimica Analitica", 3° edition, EdiSES, 2015.
- D.A. Skoog, F.J. Holler, S.R. Crouch "Chimica Analitica Strumentale", 2° edition, EdiSES, 2009.
